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KMID : 0368419910340010019
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Journal of Plant Biology
1991 Volume.34 No. 1 p.19 ~ p.23
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Isolation, Culture and Electroporation of Rice Protoplasts
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Abstract
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Culture of embryogenic callus and suspension were induced from rice seeds in MS2.5 medium. In hormone free N6 medium, whole plantlets were regenerated from embryogenic callus. We observed cell division and reformation of embryogenic callus on culture of protoplast isolated from embryogenic cell suspensions. In addition, we studied the influencing factors on viability of protoplast treated with electroporation. Viability was decreased according to the increase of voltage and capacitance during electroporation. An optimal level of viability was obtained after treatment with 200-300 V/1180 μF in HBM buffer at 4℃.
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